Use of a secretion trap screen in pepper following Phytophthora capsici infection reveals novel functions of secreted plant proteins in modulating cell death.
Identifieur interne : 001632 ( Main/Exploration ); précédent : 001631; suivant : 001633Use of a secretion trap screen in pepper following Phytophthora capsici infection reveals novel functions of secreted plant proteins in modulating cell death.
Auteurs : Seon-In Yeom [Corée du Sud] ; Hyang-Ku Baek ; Sang-Keun Oh ; Won-Hee Kang ; Sang Jik Lee ; Je Min Lee ; Eunyoung Seo ; Jocelyn K C. Rose ; Byung-Dong Kim ; Doil ChoiSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2011.
Descripteurs français
- KwdFr :
- Capsicum (microbiologie), Capsicum (métabolisme), Extinction de l'expression des gènes (MeSH), Interactions hôte-pathogène (MeSH), Maladies des plantes (microbiologie), Mort cellulaire (physiologie), Phytophthora (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (physiologie).
- MESH :
- génétique : Protéines végétales.
- microbiologie : Capsicum, Maladies des plantes.
- métabolisme : Capsicum, Protéines végétales.
- physiologie : Mort cellulaire, Phytophthora, Régulation de l'expression des gènes végétaux.
- Extinction de l'expression des gènes, Interactions hôte-pathogène.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- metabolism : Capsicum, Plant Proteins.
- microbiology : Capsicum, Plant Diseases.
- physiology : Cell Death, Gene Expression Regulation, Plant, Phytophthora.
- Gene Silencing, Host-Pathogen Interactions.
Abstract
In plants, the primary defense against pathogens is mostly inducible and associated with cell wall modification and defense-related gene expression, including many secreted proteins. To study the role of secreted proteins, a yeast-based signal-sequence trap screening was conducted with the RNA from Phytophthora capsici-inoculated root of Capsicum annuum 'Criollo de Morelos 334' (CM334). In total, 101 Capsicum annuum secretome (CaS) clones were isolated and identified, of which 92 were predicted to have a secretory signal sequence at their N-terminus. To identify differences in expressed CaS genes between resistant and susceptible cultivars of pepper, reverse Northern blots and real-time reverse-transcription polymerase chain reaction were performed with RNA samples isolated at different time points following P. capsici inoculation. In an attempt to assign biological functions to CaS genes, we performed in planta knock-down assays using the Tobacco rattle virus-based gene-silencing method. Silencing of eight CaS genes in pepper resulted in suppression of the cell death induced by the non-host bacterial pathogen (Pseudomonas syringae pv. tomato T1). Three CaS genes induced phenotypic abnormalities in silenced plants and one, CaS259 (PR4-l), caused both cell death suppression and perturbed phenotypes. These results provide evidence that the CaS genes may play important roles in pathogen defense as well as developmental processes.
DOI: 10.1094/MPMI-08-10-0183
PubMed: 21542767
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Rose</name></author><author><name sortKey="Kim, Byung Dong" sort="Kim, Byung Dong" uniqKey="Kim B" first="Byung-Dong" last="Kim">Byung-Dong Kim</name></author><author><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Capsicum (metabolism)</term><term>Capsicum (microbiology)</term><term>Cell Death (physiology)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Gene Silencing (MeSH)</term><term>Host-Pathogen Interactions (MeSH)</term><term>Phytophthora (physiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Capsicum (microbiologie)</term><term>Capsicum (métabolisme)</term><term>Extinction de l'expression des gènes (MeSH)</term><term>Interactions hôte-pathogène (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Mort cellulaire (physiologie)</term><term>Phytophthora (physiologie)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Capsicum</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Capsicum</term><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Capsicum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Capsicum</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mort cellulaire</term><term>Phytophthora</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Death</term><term>Gene Expression Regulation, Plant</term><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Silencing</term><term>Host-Pathogen Interactions</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Interactions hôte-pathogène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In plants, the primary defense against pathogens is mostly inducible and associated with cell wall modification and defense-related gene expression, including many secreted proteins. To study the role of secreted proteins, a yeast-based signal-sequence trap screening was conducted with the RNA from Phytophthora capsici-inoculated root of Capsicum annuum 'Criollo de Morelos 334' (CM334). In total, 101 Capsicum annuum secretome (CaS) clones were isolated and identified, of which 92 were predicted to have a secretory signal sequence at their N-terminus. To identify differences in expressed CaS genes between resistant and susceptible cultivars of pepper, reverse Northern blots and real-time reverse-transcription polymerase chain reaction were performed with RNA samples isolated at different time points following P. capsici inoculation. In an attempt to assign biological functions to CaS genes, we performed in planta knock-down assays using the Tobacco rattle virus-based gene-silencing method. Silencing of eight CaS genes in pepper resulted in suppression of the cell death induced by the non-host bacterial pathogen (Pseudomonas syringae pv. tomato T1). Three CaS genes induced phenotypic abnormalities in silenced plants and one, CaS259 (PR4-l), caused both cell death suppression and perturbed phenotypes. These results provide evidence that the CaS genes may play important roles in pathogen defense as well as developmental processes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21542767</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>12</Day></DateCompleted><DateRevised><Year>2011</Year><Month>05</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>6</Issue><PubDate><Year>2011</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Use of a secretion trap screen in pepper following Phytophthora capsici infection reveals novel functions of secreted plant proteins in modulating cell death.</ArticleTitle><Pagination><MedlinePgn>671-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-08-10-0183</ELocationID><Abstract><AbstractText>In plants, the primary defense against pathogens is mostly inducible and associated with cell wall modification and defense-related gene expression, including many secreted proteins. To study the role of secreted proteins, a yeast-based signal-sequence trap screening was conducted with the RNA from Phytophthora capsici-inoculated root of Capsicum annuum 'Criollo de Morelos 334' (CM334). In total, 101 Capsicum annuum secretome (CaS) clones were isolated and identified, of which 92 were predicted to have a secretory signal sequence at their N-terminus. To identify differences in expressed CaS genes between resistant and susceptible cultivars of pepper, reverse Northern blots and real-time reverse-transcription polymerase chain reaction were performed with RNA samples isolated at different time points following P. capsici inoculation. In an attempt to assign biological functions to CaS genes, we performed in planta knock-down assays using the Tobacco rattle virus-based gene-silencing method. Silencing of eight CaS genes in pepper resulted in suppression of the cell death induced by the non-host bacterial pathogen (Pseudomonas syringae pv. tomato T1). Three CaS genes induced phenotypic abnormalities in silenced plants and one, CaS259 (PR4-l), caused both cell death suppression and perturbed phenotypes. These results provide evidence that the CaS genes may play important roles in pathogen defense as well as developmental processes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yeom</LastName><ForeName>Seon-In</ForeName><Initials>SI</Initials><AffiliationInfo><Affiliation>Department of Plant Science, Seoul National University, Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baek</LastName><ForeName>Hyang-Ku</ForeName><Initials>HK</Initials></Author><Author ValidYN="Y"><LastName>Oh</LastName><ForeName>Sang-Keun</ForeName><Initials>SK</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Won-Hee</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang Jik</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Je Min</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Seo</LastName><ForeName>Eunyoung</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Rose</LastName><ForeName>Jocelyn K C</ForeName><Initials>JK</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Byung-Dong</ForeName><Initials>BD</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Doil</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="N">Capsicum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>5</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21542767</ArticleId><ArticleId IdType="doi">10.1094/MPMI-08-10-0183</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><noCountry><name sortKey="Baek, Hyang Ku" sort="Baek, Hyang Ku" uniqKey="Baek H" first="Hyang-Ku" last="Baek">Hyang-Ku Baek</name><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name><name sortKey="Kang, Won Hee" sort="Kang, Won Hee" uniqKey="Kang W" first="Won-Hee" last="Kang">Won-Hee Kang</name><name sortKey="Kim, Byung Dong" sort="Kim, Byung Dong" uniqKey="Kim B" first="Byung-Dong" last="Kim">Byung-Dong Kim</name><name sortKey="Lee, Je Min" sort="Lee, Je Min" uniqKey="Lee J" first="Je Min" last="Lee">Je Min Lee</name><name sortKey="Lee, Sang Jik" sort="Lee, Sang Jik" uniqKey="Lee S" first="Sang Jik" last="Lee">Sang Jik Lee</name><name sortKey="Oh, Sang Keun" sort="Oh, Sang Keun" uniqKey="Oh S" first="Sang-Keun" last="Oh">Sang-Keun Oh</name><name sortKey="Rose, Jocelyn K C" sort="Rose, Jocelyn K C" uniqKey="Rose J" first="Jocelyn K C" last="Rose">Jocelyn K C. Rose</name><name sortKey="Seo, Eunyoung" sort="Seo, Eunyoung" uniqKey="Seo E" first="Eunyoung" last="Seo">Eunyoung Seo</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Yeom, Seon In" sort="Yeom, Seon In" uniqKey="Yeom S" first="Seon-In" last="Yeom">Seon-In Yeom</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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